Isolation and Purification of Proteins

Isolation and Purification of Proteins

by Bo Mattiasson (Editor), RajniHatti-Kaul (Editor)

Synopsis

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

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More Information

Format: Paperback
Pages: 676
Edition: 1
Publisher: CRC Press
Published: 30 Aug 2019

ISBN 10: 0367395495
ISBN 13: 9780367395490